Development of immunodetection system for botulinum neurotoxin serotype E
نویسندگان
چکیده
منابع مشابه
Development of an immunodetection test for a botulinum-like neurotoxin produced by Clostridium sp. RKD.
BACKGROUND & OBJECTIVES Clostridial neurotoxins are among the most toxic substances known and cause severe illnesses in both humans and animals. A neurotoxigenic Clostridium sp. (strain RKD) isolated from intestine of decaying fish produced a novel, botulinum type B like neurotoxin as suggested by mouse bioassay, protection with anti-botulinum antibodies and PCR. The aim of the present investig...
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TO THE EDITOR—I write in reference to the publication of the articles and commentaries regarding the purported new botulinum neurotoxin serotype H [1–4]. For the past 30 years, the genetics and physiology of Clostridium botulinum, particularly the properties of serotypes and subtypes of botulinum neurotoxins, have been studied in my tier 1 select agent laboratory. I also have a dual use researc...
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BACKGROUND Botulinum neurotoxins (BoNTs) are considered to be the most toxic substances known on earth and are responsible for human botulism, a life-threatening disease characterized by flaccid muscle paralysis that occurs naturally by food-poisoning or colonization of the gastrointestinal tract by BoNT-producing clostridia. BoNTs have been classified as category A agent by the Centers of Dise...
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An in silico screen of the NIH Molecular Library Small Molecule Repository (MLSMR) of ∼350000 compounds and confirmatory bioassays led to identification of chaetochromin A (1) as an inhibitor of botulinum neurotoxin serotype A (BoNT A). Subsequent acquisition and testing of analogues of 1 uncovered two compounds, talaroderxines A (2) and B (3), with improved activity. These are the first fungal...
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Human botulism is most commonly caused by botulinum neurotoxin (BoNT) serotypes A, B, and E. For this work, we sought to develop a human monoclonal antibody (mAb)-based antitoxin capable of binding and neutralizing multiple subtypes of BoNT/E. Libraries of yeast-displayed single chain Fv (scFv) antibodies were created from the heavy and light chain variable region genes of humans immunized with...
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ژورنال
عنوان ژورنال: Indian Journal of Medical Research
سال: 2018
ISSN: 0971-5916
DOI: 10.4103/ijmr.ijmr_1375_16